| 摘要 |
There is disclosed a method of separating a posterior endothelial layer of a donor cornea having an epithelium, layers of stroma, a Descemet's membrane and an endothelium, after the donor cornea has been harvested from a cadaver. The method comprises the steps of mounting the donor cornea on an artificial anterior chamber provided with a datum point and means for receiving a microkeratome and using the microkeratome to separate the posterior endothelial layer of the donor cornea. The artificial anterior chamber is configured such that the datum point is located at a predetermined height relative to the endothelium when the donor cornea is mounted, and a cutting height of the microkeratome is set to a predetermined height with reference to the datum point. There is also disclosed an artificial anterior chamber and a microkeratome for use with the method.
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