BISPECIFIC ANTIBODIES AND METHODS FOR PRODUCTION THEREOF

摘要

The invention relates to an ex vivo method for the generation of a bispec ific antibody, comprising the steps of: a) providing a first antibody having a first binding specificity, wherein said first antibody comprises an IgG4- like CH3 region, b) providing a second antibody having a second binding spec ificity which differs from said first binding specificity, wherein said seco nd antibody comprises an IgG4-like CH3 region, c) incubating said first and second antibodies together under reducing conditions which allow the cystein es in the core hinge region to undergo disulfidebond isomerization, and d) o btaining a bispecific antibody. The invention furthermore relates to bispeci fic antibodies obtainable by the method of the invention.