| 摘要 |
A method for making dihydroxyacetone phosphate (DHAP), comprising treating dihydroxyacetone with a bacterial acid phosphatase in the presence of pyrophosphate. The invention further pertains to a method for stereospecifically making, preferably in one pot, a compound of the formula: <?in-line-formulae description="In-line Formulae" end="lead"?>R-C*H(OH)-C*H(OH)-CO-CH<SUB>2</SUB>-OH<?in-line-formulae description="In-line Formulae" end="tail"?> comprising the steps: a) treating dihydroxyacetone with a bacterial acid phosphatase in the presence of pyrophosphate to make dihydroxyacetone phosphate; b) treating the dihydroxyacetone phosphate with R-CHO and an aldolase, wherein R-CHO is any aldehyde, preferably R is selected from H, unsubstituted or substituted (cyclo)alkyl, and a carbohydrate moiety, and C* stands for a chiral carbon atom to a phosphate of the formula: <?in-line-formulae description="In-line Formulae" end="lead"?>R-C*H(OH)-C*H(OH)-CO-CH<SUB>2</SUB>-OPO<SUB>3</SUB>H<SUB>2</SUB>; and<?in-line-formulae description="In-line Formulae" end="tail"?> c) dephosphorylating the phosphate of step b) by treating the phosphate with a bacterial acid phosphatase.
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