发明名称 |
VERFAHREN ZUR REINIGUNG VOM RECOMBINANTEM HCG MIT SPEZIFISCHER BIOAKTIVITÄT |
摘要 |
A process for the purification of recombinant human Chorionic Gonadotropin (hCG) from a sample of crude recombinant hCG in the supernatant of CHO cells comprises the combined use of ion-exchange chromatography and reverse phase HPLC. The ion-exchange chromatography is performed twice and the final use of a size exclusion chromatography allows the purification from any residual traces of contaminants. The specific bioactivity of the highly purified hCG obtained from the process is particularly high, amounting to about 25.000 IU/mg. |
申请公布号 |
DE60135541(D1) |
申请公布日期 |
2008.10.09 |
申请号 |
DE2001635541 |
申请日期 |
2001.01.22 |
申请人 |
LABORATOIRES SERONO S.A., COINSINS |
发明人 |
PARADISI, GIANFRANCO;ROSSI, MARA;SCAGLIA, LAURA |
分类号 |
C07K1/36;G01N30/26;A61K38/24;A61P15/08;B01J20/281;B01J20/283;C07K14/59;C12N15/09;G01N30/34;G01N30/46;G01N30/88;G01N30/96 |
主分类号 |
C07K1/36 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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