METHODS AND COMPOSITIONS FOR MULTIPLEXING DETECTION OF NUCLEIC ACID SEQUENCES WITHIN AN ARRAY ELEMENT

摘要

The invention provides a method of detecting nucleic acid sequences. The method includes: (a) contacting under conditions sufficient for hybridization a population of target nucleic acids with a plurality of multiplex substrate elements, each element comprising an attached first nucleic acid and an attached second nucleic acid, said first nucleic acid comprising a first target specific probe, said second nucleic acid comprising a second target specific probe, thereby forming hybridization complexes comprising said first target specific probe with a first target nucleic acid and said second target specific probe with a second target nucleic acid, wherein said first target nucleic acid has a sequence that is different from said second target nucleic acid; (b) contacting said hybridization complexes with a polymerase and a nucleotide mixture to modify at least one of said target specific probes, thereby forming at least one modified target specific probe, said nucleotide mixture containing at least two nucleotides having first and second distinct labels, respectively, and (c) determining incorporation of said first or second label into said at least one modified target specific probe, thereby determining the presence or absence of said first or second target sequences.