FLUORESCENT LABELED FUSED PROTEIN FOR ASSAYING ADENOSINE TRIPHOSPHATE

摘要

It is intended to provide a substance which makes it possible to highly accurately assay ATP by a convenient procedure without depending on a protein concentration. It is also intended to provide a method of assaying ATP using this substance. These objects can be achieved by a fluorescent labeled fused protein, wherein two fluorescent substances respectively serving as a donor and an acceptor in fluorescence resonance energy transfer (FRET) are individually bonded to a protein capable of undergoing a structural change depending on the binding to ATP (i.e., an e protein that is a subunit of ATP synthase), and a method which comprises bringing this fluorescent labeled fused protein into contact with a test substance and then measuring the fluorescence spectrum.