| 摘要 |
PURPOSE:To make it possible to achieve the compact configuration, high performance, easy manufacturing and the like of an enzyme electrode by obtaining the fact that a conductive macromolecular film is oxidized or reduced with the product due to enzyme reaction in an immobilized enzyme film and the conductivity is changed, and detecting the concentration of the biochemical material in a sample. CONSTITUTION:A metallic thin film 3 is formed on an insulating substrate 2 as electrode metal by vacuum vapor deposition. A groove 4 is formed in the film 3, and electrodes 3a and 3b are separated. Lead wires 7 and 7 are connected. Furthermore, an electrode (exposed) surface 3c is determined, and the other part is covered with an insulating film 5. Then, a polyaniline film (conductive macromolecular film) 8 is formed by electrolytic polymerization. Glucose oxidase is fixed on the polyaniline film 8, and a fixed layer 9 is formed. Reaction due to the glucose oxidase occurs in the fixed layer 9. The polyaniline film 8 is oxidized with H2O2 which is the product. Thus the concentration of the glucose can be detected from the change in conductivity. |
