| 摘要 |
<p>Method for label-free sequencing of nucleic acid-single strand comprises (a) incubating solid phase immobilized DNA-probes with single stranded nucleic acid analytes for forming a hybridizing complex; (b) adding one of the four nucleoside; (c) inducing the nucleoside triphosphate to release pyrophosphate ions; (d) removing excess nucleoside triphosphate; (e) repeating the steps (b)-(d); optionally (f) multiple repeating of the steps (b)-(e); and (g) determining the nucleic acid sequence analyte based on the measured data recorded in the step (c). Method for label-free sequencing of nucleic acid-single strand, in which the DNA-polymerase or a functional fragment is used, comprises (a) incubating solid phase immobilized DNA-probes with single stranded nucleic acid analytes for forming a hybridizing complex; (b) adding one of the four nucleoside triphosphate dATP, deoxycytidine triphosphate, deoxyguanosine triphosphate or deoxythymidine triphosphate; (c) inducing the nucleoside triphosphate in the presence of the DNA-polymerase or its fragment to release pyrophosphate ions and detected by a sensor; (d) removing excess nucleoside triphosphate; (e) repeating the steps (b)-(d) under the addition of other nucleoside triphosphate; optionally (f) multiple repeating of the steps (b)-(e); and (g) determining the nucleic acid sequence analyte based on the measured data recorded in the step (c); where the detection in step (c) is carried out with at least a sensor, which is monolithically integrated in the solid phase.</p> |
