| 摘要 |
<p>A method for forming glucose sensing micro-particles is provided. The method comprises the steps of (a) providing a suspension comprising hydrogel micro-particles, the hydrogel micro-particles comprising a covalently cross-linked or ionically cross-linked hydrogel matrix which comprises functional groups; (b) adding a reagents to the suspension to activate the functional groups of the hydrogel micro-particles; and (c), after having activated the functional groups, adding a glucose-binding lectin, such as concanavalin A in pure or modified form, a stabilised glucose-specific enzyme, an appropriate molecule from aptamer or spiegelmer classes, or a mixture thereof to the suspension to link the glucose-binding lectin, stabilised glucose-specific enzyme, or molecule from aptamer or spiegelmer classes covalently to the activated functional groups.</p> |
