Use of an autologous culture medium comprising autologous human serum and nutrients, together with an anticoagulant, allows the in vitro culture, purification and expansion of autologous human progenitor stem cells

摘要

Provided is a method for the preparation of autologous culture medium of autologous human progenitor stem cells which comprises: a) between 0.1 % and 90% weight of autologous human serum; b) between 0.1 and 10,000 UI/ml heparin, c) between 0.1 and 10,000 UI/ml protamine; and d) a culture medium with basic nutrients with or without glutamine, in sufficient quantity up to 100% weight, which comprises mixing together autologous human serum, heparin, protamine, basic nutrients with or without glutamine. Further provided are similar methods for preparing a culture medium containing additionally specific antibiotics and use of the media for culturing said stem cells and for obtaining said stem cells from muscle or blood cells as well as pharmaceutical compositions comprising said cell culture. Also provided is the use of such stem cells in the manufacture of a medicament to treat dysfunctional myocardial tissue.