Remodeling and glycoconjugation of peptides

摘要

Disclosed is a covalent conjugate between a polymeric modifying group and a glycosylated or non-glycosylated peptide, wherein said polymeric modifying group is conjugated to said peptide via an intact glycosyllinking group, wherein said glycosyllinking group is interposed between and covalently linked to both said peptide and said polymeric modifying group, wherein said covalent conjugate is formed by contacting said peptide with at least one glycosyltransferase and at least one modified sugar donor under conditions suitable for said at least one glycosyltransferase to transfer a modified sugar moiety of said at least one modified sugar donor to said peptide, thereby forming said covalent conjugate, and wherein said modified sugar moiety comprises said polymeric modifying group.