| 摘要 |
A method for selecting a G-protein coupled receptor (GPCR) with increased stability, the method comprising providing one or more mutants of a parent GPCR, selecting a ligand, the ligand being one which binds to the parent GPCR when the GPCR is residing in a particular conformation, determining whether the or each mutant GPCR has increased stability with respect to binding the selected ligand compared to the stability of the parent GPCR with respect to binding that ligand, and selecting those mutants that have an increased stability compared to the parent GPCR with respect to binding the selected ligand. Mthods of producing such proteins are also claimed as are mutants of P-adrenergic receptor, adenosine receptor, neurotensin receptor and muscarine receptor. These proteins have increased heat, detergent, chaotropic of pH stability, most preferably increased thermostability. |
