发明名称 |
Utilization of nucleotide probes in ELISA procedure for the quantitative determination of Plasmodium falciparum DNA in malaria |
摘要 |
The present invention is the development of a simple and specific quantitative method for the determination of P. falciparum DNA in malaria that involves the direct detection of the highly 42-kDa conserved C-terminal region of P. falciparum merozoite surface protein (MSP1) gene. This procedure entails the amplification of the 42-kDa C-terminal region of the MSP1 gene by using the PCR technique in the presence of digoxigenin-11-dUTP and the synthesis of the specific biotin labeled nucleotide probes directed to the 42-kDa C-terminal region of the MSP1 gene. These specific probes are then used in the Enzyme Linked Immunosorbent Assay (ELISA) for the quantitative determination of the 42-kDa C-terminal region of the MSP1 gene which leads to the quantitative determination of P. falciparum DNA in malaria for quantitative diagnostic purpose as well as for monitoring the efficacy of antimalarial treatment.
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申请公布号 |
US7427483(B2) |
申请公布日期 |
2008.09.23 |
申请号 |
US20060307852 |
申请日期 |
2006.02.24 |
申请人 |
VISTA BIOLOGICALS CORPORATION |
发明人 |
NGUYEN KHUE VU |
分类号 |
C12Q1/68;C07H21/04;C12P19/34;G01N33/00 |
主分类号 |
C12Q1/68 |
代理机构 |
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