STRAIN E coli BL21(DE3)/pET-hHSP70 - PRODUCENT OF PROTEIN OF HEAT SHOCK IN HUMANS HSP70

摘要

FIELD: chemistry. ^ SUBSTANCE: invention relates to the field of biotechnology and can be used in medico-biological industry. On the basis of vector pET28a(+) plasmid DNA pET-hHSP70, which contains nucleotide sequence encoding protein of heat shock in humans hHSP70A1B is constructed. By transforming cells of E.coli by plasmid DNA pET-hHSP70 strain of E.coli BL21(DE3)/pET-hHSP70 - producent of recombinant form hHSP70 - is obtained. ^ EFFECT: obtaining recombinant protein hHSP70 with a high output (in amount of 30-40% of total content of cell protein) and simplifying its isolation and purification. ^ 3 dwg, 3 ex