发明名称 |
A new DNA polymerase with proof-reading 3'-5' exonuclease activity |
摘要 |
<p>The invention relates to DNA polymerases which are capable of proofreading 3'-5' exonuclease activity during DNA sequencing of a DNA strand, such that the DNA polymerase functions to excise mismatched nucleotides from the 3' terminus of the DNA strand at a faster rate than the rate at which the DNA polymerase functions to remove nucleotides matched correctly with the nucleotides of the template, and which DNA polymerase does not exhibit 5'-3' exonuclease activity. The invention also relates to isolated and cloned DNA sequences derived from the Bacillus stearothermophilus thermostable DNA polymerase, as well as the expressed polymerase itself.</p> |
申请公布号 |
EP0810288(A2) |
申请公布日期 |
1997.12.03 |
申请号 |
EP19960303375 |
申请日期 |
1996.05.14 |
申请人 |
HONG, GUO FAN;ZHAI, FENG;HUANG, WEI-HUA |
发明人 |
HONG, GUO FAN;ZHAI, FENG;HUANG, WEI-HUA |
分类号 |
C12N15/09;C07H21/04;C12N1/21;C12N9/12;C12N15/00;C12N15/54;C12N15/55;C12Q1/68;C12R1/07;C12R1/19;(IPC1-7):C12N15/55;C12N9/22;C12N15/70 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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