FLUORESCENCE-BASED ASSAY FOR DETECTING COMPOUNDS FOR MODULATING THE SODIUM-CALCIUM EXCHANGER (NCX) IN ~FORWARD MODE~

摘要

Carriers are an up-and-coming target family with enormous potential and o ffer commercial and scientific possibilities. The sodium/calcium exchanger i s an important mechanism for the removal of Ca2+ from various cells. In the heart, said sodium/calcium exchanger extrudes Ca2+, which has entered throug h Ca2+ channels for the initiation of contractions, while Na+ enters the hea rt cell. It is of considerable interest to identify compounds that modulate the activity of the sodium-calcium exchanger. The present invention relates to a fluorescence-based assay for the detection of compounds for modulating the NCX in ~forward mode.~ The invention further relates to a parts set comp rising cells that express NCX and to the use of said parts set for testing a compound for activity as an agonist or antagonist of NCX.