| 摘要 |
A method for identifying the agonistic activity of a target compound on a potassium channel by a) providing a population of cells expressing a potassium channel and, optionally providing, a protein-based fluorescent-optical voltage sensor, b) if necessary, incubating the cells with a voltage-sensitive fluorescent dye, c) adding the target compound to the reaction batch of a) or b), d) determining a value F<SUB>1 </SUB>of the fluorescence intensity of the cells, e) adding potassium ions in a physiologically acceptable concentration f) determining a value F<SUB>2 </SUB>of the fluorescence intensity of the cells, and g) comparing the fluorescence intensity F<SUB>2 </SUB>with the fluorescence intensity F<SUB>1 </SUB>and determining the agonistic activity of the target compound on a potassium channel therefrom. A potassium channel agonist isolated and purified by this method, pharmaceutical formulations containing the same, and their use for the treatment of a disease in which potassium channels are involved.
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