发明名称 |
Fluorescence polarization-based homogeneous assay for deoxynivalenol determination in grains |
摘要 |
<p>The invention relates to a homogeneous assay for determining the deoxynivanlenol (DON) content in grains wherein the technique of fluorescence polarization is used. A grain extract is prepared by shaking a crushed grain sample with water. A mixture is prepared by combining the grain extract with a tracer and with monoclonal antibodies specific to DON. The tracer is able to bind to the monoclonal antibodies to produce a detectable change in fluorescence polarization. The tracer is prepared by conjugating DON to a suitable fluorophore. The fluorescence polarization of the mixture is measured. The DON concentration of the mixture may be calculated using a standard curve obtained by measuring the fluorescence polarization of a series of DON solutions of known concentration.</p> |
申请公布号 |
EP1881324(A3) |
申请公布日期 |
2008.09.17 |
申请号 |
EP20070021724 |
申请日期 |
2001.09.10 |
申请人 |
DIACHEMIX LLC |
发明人 |
NASIR, MOHAMMAD SARWAR;JOLLEY, MICHAEL E. |
分类号 |
G01N33/542;G01N33/569 |
主分类号 |
G01N33/542 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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