发明名称 Polymerase stabilization by ionic detergents
摘要 <p>The invention relates to a composition comprising (i) an enzyme with nucleic acid polymerase activity, (ii) an inert protein and, (ii) an ionic detergent. The invention relates also to a composition comprising (i) an enzyme with nucleic acid polymerase activity, (ii) an inert protein and, (ii) a zwitterionic detergent. The invention further relates to a method for enzymatic nucleic acid synthesis comprising the steps of, (a) providing in a reaction mixture, a polymerase activity, a nucleic acid template, a ionic detergent, preferably zwitterionic detergent, a buffer, a salt, nucleotides and an inert protein and, (b) incubating the reaction mixture at a temperature which enables nucleic acid synthesis.</p>
申请公布号 EP1970440(A1) 申请公布日期 2008.09.17
申请号 EP20070103648 申请日期 2007.03.06
申请人 QIAGEN GMBH 发明人 FANG, NAN;LOEFFERT, DIRK;ERBACH, CHRISTOPH;PETERS, LARS-ERIK
分类号 C12N9/10 主分类号 C12N9/10
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