发明名称 |
REGIO- AND ENANTIOSELECTIVE ALKANE HYDROXYLATION WITH MODIFIED CYTOCHROME P450 |
摘要 |
<p>Cytochrome P450 BM-3 from Bacillus megaterium was engineered using a combination of directed evolution and site-directed mutagenesis to hydroxylate linear alkanes regio- and enantioselectively using atmospheric dioxygen as an oxidant. Mutant 9-10A-A328V hydroxylates octane primarily at the 2-position to form S-2-octanol (40% ee). Another mutant, 1-12G, hydroxylates alkanes larger than hexane primarily at the 2-position, but forms R-2-alcohols (40-55% ee). These biocatalysts are highly active for alkane substrates and support thousands of product turnovers. These regio- and enantio-selectivities are retained in whole-cell biotransformations with E. coli, where the engineered P450s can be expressed at high levels and the expensive cofactor is supplied endogenously.</p> |
申请公布号 |
EP1639091(A4) |
申请公布日期 |
2008.09.17 |
申请号 |
EP20040776528 |
申请日期 |
2004.06.15 |
申请人 |
CALIFORNIA UNIVERSITY OF TECHNOLOGY |
发明人 |
ARNOLD, FRANCES, H.;PETERS, MATHEW W.;MEINHOLD, PETER |
分类号 |
C12N9/00;A61K38/44;C07H21/04;C07K1/00;C07K16/00;C12N;C12N1/20;C12N9/02;C12N15/00;C12P21/04;C12P21/06;C12Q1/00;C12Q1/34;C12Q1/68 |
主分类号 |
C12N9/00 |
代理机构 |
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代理人 |
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地址 |
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