| 摘要 |
The invention provides a compact optical scanning observation apparatus having an internal focusing mechanism and which is suitable for in-vivo observation of animals. The optical scanning observation apparatus comprises a light source (2) for illuminating a sample (A); an objective optical system (10) for focusing illumination light onto the sample; a detection-light splitting device (12) for splitting off collected detection light from the illumination light; a detection-light focusing optical system (13,13') for focusing the split-off detection light; a detector (11) for detecting the focused detection light; a focus scanning device (6,6') disposed between the detection-light splitting device and the objective optical system; and a lateral-direction scanning device (7), disposed between the focus scanning device and the objective optical system, for scanning the illumination light from the light source on the sample, in substantially orthogonal directions with respect to the optical axis. The focus scanning device includes a focusing optical system formed of at least a positive lens group (G 1 ) and a negative lens group (G 2 ), and a lens driving device (6a) for moving at least one lens group included in the focusing optical system to change a working distance (WD) of the objective optical system.
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