发明名称 |
Method for screening peptides for use in immunotherapy |
摘要 |
Identifying subdominant/cryptic epitopes (I) that are presented by a HLA (human leukocyte antigen) Class I molecule, is new. Identifying subdominant/cryptic epitopes (I) that are presented by a HLA (human leukocyte antigen) Class I molecule comprising selecting at least one peptide (II) of 8-11 amino acids (aa), potentially representing an epitope for Class I presentation, from a protein against which a cytotoxic T cell (CTL) response is to be raised. (II) corresponds to a non-immunogenic peptide with low affinity for Class I molecules. Variants (IIa) of (II) are prepared in which the N-terminal aa is replaced by Tyr and their immunogenicity detected by identifying those that generate a CTL response against target cells expressing the parent protein. Peptide sequences from which active (IIa) are derived are then identified. Independent claims are also included for the following: (1) immunogenic peptide epitopes (IIa) derived from (I) identified this way; and (2) nucleic acid (III) that encodes chimeric polypeptides (IV) containing one or more, same or different, copies of (IIa).
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申请公布号 |
US7425606(B2) |
申请公布日期 |
2008.09.16 |
申请号 |
US20030333430 |
申请日期 |
2003.10.02 |
申请人 |
INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE (INSERM);INSTITUT GUSTAVE ROUSSY (IGR) |
发明人 |
KOSMATOPOULOS KOSTAS;TOURDOT SOPHIE;SCARDINO ANTONIO;GROSS ALEXANDRE DAVID |
分类号 |
C07K2/00;A61P31/12;A61P35/00;C07K14/16;C07K14/71;C12N9/12;G01N33/569 |
主分类号 |
C07K2/00 |
代理机构 |
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