Contaminant particle identification and compensation with multichannel information during scanner calibration

摘要

Methods of identifying the location and dimensions of a contaminant particle on a calibration strip comprise imaging a calibration strip comprising a plurality of channels and a plurality of pixel columns. The method also includes generating a normalized local average pixel intensity value and locating a contaminant particle in a pixel column by comparing the intensity of an individual pixel in the pixel column to the normalized local average pixel intensity value. An intensity value of an individual pixel less than a predefined limit set below the normalized local average pixel intensity value corresponds to a location of a contaminant particle. The method further includes identifying the number of adjacent pixels in a pixel column less than a predefined limit set below the normalized local average pixel intensity value.