发明名称 |
IN SITU ASSEMBLY OF PROTEIN MICROARRAYS |
摘要 |
The invention provides a microarray and methods for producing a protein microarray. The array comprises multiple nucleic acid molecules immobilized on a substrate, each comprising (i) a protein-binding domain and (ii) a nucleic acid sequence encoding a fusion protein comprising a polypeptide of interest and a DNA-binding protein that binds the protein-binding domain, and one or more fusion proteins produced from the multiple nucleic acid molecules. Each fusion protein is immobilized on the substrate via binding to a nucleic acid sequence comprising the protein-binding domain present on the nucleic acid molecule from which the fusion protein is produced or on the substrate. The invention also provides a method of analyzing protein interactions with, for example, other proteins, lipids and drugs.
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申请公布号 |
US2008214408(A1) |
申请公布日期 |
2008.09.04 |
申请号 |
US20080105636 |
申请日期 |
2008.04.18 |
申请人 |
GOVERNMENT OF THE UINTED STATES OF AMERICA, REPRESENTED BY THE SECRETARY DEPT. |
发明人 |
CHATTERJEE DEB K.;SITARAMAN KALAVATHY;HARTLEY JAMES L.;BAPTISTA CASSIO;MUNROE DAVID J. |
分类号 |
C40B30/04;C40B40/08;C40B50/14 |
主分类号 |
C40B30/04 |
代理机构 |
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主权项 |
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地址 |
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