| 摘要 |
Apparatus and methods for measuring the fluorescence of large multi-cellu lar organisms in a sample (102, 804) of liquid includes a pumping mechanism, a fluorescence measuring device (104), a method of analyzing (100, 300, 508 ) the measurements, and optionally, a sorting mechanism (502). The pumping m echanism transfers large multi-cellular organisms from a reservoir (402, 836 ) through a fluorescence-measuring device (104) causing minimum physical dam age and/or stress. The pressure differential driving the organisms from a sa mple container/reservoir through the measuring device can be derived from gr avity (800), air pressure (412), or liquid pressure, or some combination of the three. The fluorescence can be measured in a cytometer using a light det ector or imager (106). Generally the detection element will include a filter (114), isolating the wavelength of fluorescent emission. The illumination m ay be provided by a laser (112) or by an LED (302), combined with the use of dichroic mirrors to allow multiple wavelength simultaneous illumination. In addition, an LED will typically be used with a filter to isolate the optimu m excitation wavelength while blocking scattered/reflected LED light from th e detection element (but not the fluorescence).
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