MDCK cell lines supporting viral growth to high titers and bioreactor process using the same

摘要

The invention relates to a method for eliminating DNA contaminants from a viral preparation comprising: (a) passing the viral preparation over an affinity chromatography media under conditions wherein the DNA contaminants are not retained on the affinity chromatography media and the virus present in the viral preparation are retained; (b) washing the affinity chromatography media to remove DNA contaminants; and (c) eluting the virus present in the viral preparation from the affinity chromatography media.