The invention provides methods for identifying and isolating cells that express high levels of protein. A method is described for identifying a cell or cell line expressing a desired protein comprising determining in a cell or cell line that has been transfected with a vector comprising a nucleotide sequence encoding the desired protein the intracellular expression levels of elements of the vector and also determining expression of the desired protein on the surface of the cell or cell line, in certain cases in a single assay.
申请公布号
WO2008073166(A3)
申请公布日期
2008.09.04
申请号
WO2007US18715
申请日期
2007.08.23
申请人
XCELLEREX, INC.;BREZINSKY, SHARI;HODGE, GEOFFREY, L.