ANTHER-SPECIFIC CDNA SEQUENCES, GENOMIC DNA SEQUENCES AND RECOMBINANT DNA SEQUENCES

摘要

cDNA sequences are disclosed which are expressed specifically in the anther of a plant. Genomic DNA sequences corresponding to the cDNA clones are obtained using t he cDNA clones as hybridization probes. Recombinant, or chimeric, DNA sequences are constructed in which the promoter sequence from anther-specific genomic clon es are operatively linked to a DNA sequence coding for a desired polypeptide. Transgenic plants are made in which the chimeric DNA sequences are expressed in the anther of the transgenic plant. In a preferred embodiment, the coding DNA sequence express es a polypeptide which will disrupt formation of viable pollen, resulting in a ma le- sterile plant.