Directed evolution method

摘要

A method for increasing concentration of a nucleic acid molecule, said method comprising:
(a) forming a heat-stable, water-in-oil emulsion comprising encapsulated aqueous microcompartments having a density of greater than 10 9 microcompartments per ml;
(b) including within a plurality of said microcompartments a nucleic acid and an aqueous solution comprising components necessary to perform nucleic acid amplification by the polymerase chain reaction, said components including a thermostable polymerase; and
(c) conducting thermal cycling to effect amplification of said nucleic acid to produce amplified copies of said nucleic acid, thereby increasing the concentration of said nucleic acid.