发明名称 |
CHROMOSOME CONFORMATION CAPTURE-ON-CHIP (4C) ASSAY |
摘要 |
The present invention relates in one aspect to a method for analyzing the frequency of interaction of a target nucleotide sequence with one or more nucleotide sequences of interest (e.g., one or more genomic loci) comprising the steps of: (a) providing a sample of cross-linked DNA; (b) digesting the cross-linked DNA with a primary restriction enzyme; (c) ligating the cross-linked nucleotide sequences; (d) reversing the cross linking; (e) digesting the nucleotide sequences with a secondary restriction enzyme; (f) ligating one or more DNA sequences of known nucleotide composition to the available secondary restriction enzyme digestion site(s) that flank the one or more nucleotide sequences of interest; (g) amplifying the one or more nucleotide sequences of interest using at least two oligonucleotide primers, wherein each primer hybridises to the DNA sequences that flank the nucleotide sequence of interest; (h) hybridising the amplified sequence(s) to an array; and (i) determining the frequency of interaction between the DNA sequences. |
申请公布号 |
EP1899488(A2) |
申请公布日期 |
2008.03.19 |
申请号 |
EP20060795293 |
申请日期 |
2006.07.03 |
申请人 |
ERASMUS UNIVERSITY MEDICAL CENTER |
发明人 |
GROSVELD, FRANK;DE LAAT, WOUTER |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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