Fret probes and methods for detecting interacting molecules

摘要

Disclosed is a method for detecting at least two interacting molecules in a cell using a set of at least a first and a second molecular probe, each probe provided with a dye wherein said dyes together allow energy transfer and each probe comprising a binding domain capable of specifically binding to a different molecule of interest, wherein at least one probe is provided with a reactive group that is not involved in binding to a molecule of interest but allows for juxtaposing said at least first and second probe following binding to said molecule of interest such that there is an increased likelihood of energy transfer between dyes, wherein a reactive group of said first probe is not directly reactive with said second probe.