发明名称 |
Method and apparatus for transferring growth media and infection fluids to a cell growth bag |
摘要 |
A method and apparatus are provided for transferring growth media or infection fluids to a bioculture bag having Could Process fitting thereon in fluid communication with the inside of the bag. The media or fluid is placed in a flask. A cap is threaded onto the neck of the flask. The cap has a spout with an opening in a distal end of the spout and a vent hole in the cap. The spout has a tubular end in which the opening is formed, with an O-ring seal adjacent the distal end of the tubular end. The tubular end fits within the fitting and the seal forms a fluid tight seal with the fitting to allow fluid transfer to the bag in reduced time with reduced spillage. The cap is preferably used on a flask having shaped baffles in the bottom. The baffles are highest toward the centerline, and decline in height linearly toward the corners of the flask at an angle of about 9° to the horizontal. The flask is rotated about 80-180 RPM, and the baffles have leading and trailing sides in the direction of rotation that are inclined at about 32° relative to the vertical. A growth media is also provided to culture the cells in the flask. |
申请公布号 |
GB2433255(B) |
申请公布日期 |
2008.08.27 |
申请号 |
GB20060024159 |
申请日期 |
2006.12.04 |
申请人 |
SAMUEL ANDREW ELLIS;KENNETH EDWARD HOLMES |
发明人 |
SAMUEL ANDREW ELLIS;KENNETH EDWARD HOLMES |
分类号 |
B65D25/40;B01L3/00;B65B3/04;B65D25/48;B65D47/06;B65D51/16 |
主分类号 |
B65D25/40 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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