| 摘要 |
FIELD: chemistry; biochemistry. ^ SUBSTANCE: present invention pertains to molecular biology and can be used for analysing nucleic acids. In the method of comparing sequences of two nucleic acids, constituting an NA-sample and an NA-target, a biochip is used, containing several single-stranded nucleic acids (NA-antisamples), which are hybridised with NA-samples. For this purpose, the biochip is made and put into a compartment, in which there is subsequent provision for denaturing conditions and conditions for movement of the NA-sample into the compartment, containing the NA-target. In the compartment with the NA-target, there is subsequent provision for conditions for hybridisation of the NA-sample with the complementary NA-target and conditions for electrophoretic movement of the NA-sample into the compartment with the biochip. Complementarity of the NA-sample and NA-target is established either on formation of a complex of the NA-target and NA-sample, or on formation in the other compartment for the NA-antisample, of a complex of NA-antisample and NA-sample, obtained by denaturisation of the complex of NA-sample and NA-target. The device for operation with the biochip, containing NA-antisamples, which are hybridised with NA-samples, comprises a compartment for accommodating the biochip and a compartment for the NA-target, joined by a channel, which is filled with medium, impermeable for the NA-target but permeable for the NA-sample. Use of the invention increases sensitivity and efficiency of analysing nucleic acids when using biochips. ^ EFFECT: increased sensitivity and efficiency of analysing nucleic acids when using biochips. ^ 20 cl, 25 dwg |
