摘要 |
A method for obtaining differentiated cells from stem cells in vitro, comprising the steps of: (a) growing stem cells in a culture medium adhered to a microcarrier or a bead, confined within a medium permeable barrier or entrapped; (b) transferring the cells in step (a) from one culture medium to another; (c) optionally repeating step (b) as required; and (d) obtaining the differentiated cells. |