IMPROVED PRODUCTION OF CLAVULANIC ACID BY GENETIC ENGINEERING OF STREPTOMYCES CLAVULIGERUS
摘要
Genetically engineered Streptomyces clavuligerus strains with improved capabilities to produce clavulanic acid are provided. The strains are genetically engineered by disrupting newly identified glyceraldehyde-3-phosphate dehydrogenase (GAPDH) genes. This results in an increased intracelluar pool of the clavulanic acid precursor D-glyceraldehyde-3-phosphate (D-G3P), and increased clavulanic acid production. Clavulanic acid production may be further increased by supplying arginine to the medium in which the S. clavuliger
申请公布号
WO2007030772(A3)
申请公布日期
2008.08.21
申请号
WO2006US35172
申请日期
2006.09.08
申请人
THE JOHNS HOPKINS UNIVERSITY;TOWNSEND, CRAIG, A.;LI, RONG-FENG