SINGLE CELL ANALYSIS BY POLYMERASE CYCLING ASSEMBLY

摘要

The invention provides a method of making measurements on individual cells of a population, particularly cells that have identifying nucleic acid sequences, such as lymphoid cells. In one aspect, the invention provides a method of making multiparameter measurements on individual cells of such a population by carrying out a polymerase cycling assembly (PCA) reaction to link their identifying nucleic acid sequences to other cellular nucleic acids of interest. The fusion products of such PCA reaction are then sequenced and tabulated to generate multiparameter data for cells of the population.

主权项

1. A method of analyzing a plurality target nucleic acids in each cell of a population, the method comprising the steps of:
providing multiple reactors each containing a single cell in a polymerase cycling assembly (PCA) reaction mixture comprising a pair of outer primers and one or more pairs of linking primers specific for the plurality of target nucleic acids; performing a PCA reaction in the reactors to form fusion products of the target nucleic acids in the reactors; and sequencing the fusion products from the reactors to identify the target nucleic acids of each cell in the population.