发明名称 |
PREPARATION OF NUCLEIC ACID TEMPLATES FOR SOLID PHASE AMPLIFICATION |
摘要 |
The invention relates to a method of preparing template constructs for solid-phase nucleic acid amplification and to use of the templates in methods of solid-phase nucleic acid amplification. The method involves carrying out two ligation reactions: (a) a ligation reaction in which the first end of one or more target polynucleotide molecules are ligated to surface-bound adaptor polynucleotide molecules, and (b) a ligation reaction in which solution-phase adaptor polynucleotide molecules are ligated to the second end of said target polynucleotide molecules, in order to produce one or more template constructs attached to a solid support. |
申请公布号 |
EP1957668(A1) |
申请公布日期 |
2008.08.20 |
申请号 |
EP20060808675 |
申请日期 |
2006.11.24 |
申请人 |
SOLEXA LTD. |
发明人 |
GORMLEY, NIALL ANTHONY;BOUTELL, JONATHAN, MARK;TURCATTI, GERADO;BARNES, COLIN LLOYD |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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