发明名称 |
Expression of G protein coupled receptors in yeast |
摘要 |
Disclosed is a transformed yeast cell containing a first heterologous DNA sequence which codes for a mammalian G protein-coupled receptor and a second heterologous DNA sequence which codes for a mammalian G protein alpha subunit (mammalian G<SUB>alpha</SUB>). The first and second heterologous DNA sequences are capable of expression in the cell, but the cell is incapable of expressing an endogenous G protein alpha-subunit (yeast G<SUB>alpha</SUB>). The cells are useful for screening compounds which affect the rate of dissociation of G<SUB>alpha</SUB> from G<SUB>betagamma</SUB> in a cell. Also disclosed is a novel DNA expression vector useful for making cells as described above. The vector contains a first segment comprising at least a fragment of the extreme amino-terminal coding sequence of a yeast G protein-coupled receptor. A second segment is positioned downstream from the first segment (and in correct reading frame therewith), with the second segment comprising a DNA sequence encoding a heterologous G protein-coupled receptor.
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申请公布号 |
US7413876(B2) |
申请公布日期 |
2008.08.19 |
申请号 |
US20040997069 |
申请日期 |
2004.11.23 |
申请人 |
DUKE UNIVERSITY OFFICE OF SCIENCE AND TECHNOLOGY |
发明人 |
KING KLIM;DOHLMAN HENRICK G.;CARON MARC G.;LEFKOWITZ ROBERT J. |
分类号 |
C12N1/19;C07K14/39;C07K14/47;C07K14/705;C12N1/18;C12N15/09;C12N15/12;C12N15/74;C12N15/81;C12P21/02;C12Q1/00;C12Q1/02;C12Q1/68;C12R1/865;G01N33/566 |
主分类号 |
C12N1/19 |
代理机构 |
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地址 |
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