发明名称 |
CODON OPTIMIZED SYNTHETIC PLASMIDS |
摘要 |
One aspect of the current invention is an optimized synthetic mammalian expression plasmid (e.g. pAV0201). This new plasmid comprise a therapeutic element, and a replication element. The therapeutic element of the new plasmid comprises a eukaryotic promoter; a 5' untranslated region ("UTR"); a codon-optimized-eukaryotic therapeutic gene sequence; and a poly adenylation signal. The therapeutic elements of this plasmid are operatively linked and located in a first operatively-linked arrangement. Additionally, the optimized synthetic mammalian expression plasmid comprises replication elements, wherein the replication elements are operatively linked and located in a second operatively-linked arrangement. The replication elements comprise a selectable marker gene promoter, a ribosomal binding site, and an origin of replication. The first-operatively-linked arrangement and the second-operatively-linked arrangement comprise a circular structure of the codon optimized synthetic mammalian expression plasmid.
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申请公布号 |
US2008194511(A1) |
申请公布日期 |
2008.08.14 |
申请号 |
US20070941876 |
申请日期 |
2007.11.16 |
申请人 |
VGX PHARMACEUTICLAS, INC. |
发明人 |
DRAGHIA-AKLI RUXANDRA;ABRUZZESE RONALD V.;KERN DOUGLAS R. |
分类号 |
A61K31/711;A61K48/00;C07K14/60;C12N15/67;C12N15/85 |
主分类号 |
A61K31/711 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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