发明名称 |
Method for the High-Speed Detection and/or Measurement of a Lipase or Phospholipase Activity |
摘要 |
The invention relates to a method for the detection and/or measurement in vitro of a lipase or phospholipase activity, characterized in that it comprises: the addition of a sample likely to contain said lipase or phospholipase into the wells of microtitration plates coated with a layer of a lipid substrate which is able to be hydrolyzed by said lipase or phospholipase by releasing alpha-eleostearic acid, the detection and/or the measurement of the lipase or phospholipase activity by UV spectrophotometry of the alpha-eleostearic acid released during the previous stage. The invention also relates to the application of the above-mentioned method to the in vitro diagnosis of pathologies linked to an increase in the plasma lipase level.
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申请公布号 |
US2008193958(A1) |
申请公布日期 |
2008.08.14 |
申请号 |
US20060884139 |
申请日期 |
2006.02.10 |
申请人 |
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE |
发明人 |
VERGER ROBERT;SERVEAU-AVESQUE CAROLE;CHAHINIAN HENRI |
分类号 |
C12Q1/00;C12M1/34;C12M3/00 |
主分类号 |
C12Q1/00 |
代理机构 |
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主权项 |
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地址 |
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