METHOD OF OBTAINING CELL LINES IN A PROTEIN-FREE MEDIUM AND CELL LINES THUS OBTAINED

摘要

The invention relates to a method of obtaining mammal cells which are adapted to grow in a medium free of serum and proteins. The method includes a process consisting of two adaptation steps comprising a critical protein concentration interval, in which the cells must acquire the ability to survive in a protein-free medium. There is a specific critical protein concentration interval for each cell line.