发明名称 |
METHOD OF PRODUCTION OF LIVE CULTURE INFLUENZA VIRUS VACCINES |
摘要 |
FIELD: medicine. ^ SUBSTANCE: invention refers to biotechnology. Method includes cultivation of transplantable cells MDCK in nutrient medium, production of virus-containing substances by cultivation vaccinating influenza virus strains in MDCK cells culture on the same nutrient medium, virus substance purification from ballast impurities, stabilising additives introduction to purified substance and drying of the finished vaccine form. Thus inoculation dose of transplantable cells MDCK is 100-600 thousand cells per 1 ml of environment. Vaccinating cold-adapted reasortants of influenza virus strains are used as virus strains with inoculation dose of infection multiplicity 0.01-0.0001 "50/-". Serum-free nutrient medium added with soya hydrolysate concentrated 0.01-10% and proteolytic enzyme in amount 0.25-50.0 mkg/ml prior to introduction of influenza virus strains inoculation dose is used as nutrient medium for cultivation of MDCK cells and influenza virus strains on specified cells. Cultivation of MDCK cells cultures and cold-adapted influenza virus strains in specified cell culture is performed in suspensions on microcarriers brought in serum-free nutrient medium in concentration 1-6 g/l. Collection of virus-containing liquid is carried out after specific activity of influenza virus is not less than 8.0 lg ""50/ml. Saccharose and soya peptone in final concentration (5-10) and (3-8) mass % respectively are used as stabilising additives introduced into purified substance before drying. ^ EFFECT: method enables to produce vaccine with lower content of foreign animal protein components. ^ 7 cl, 3 tbl, 7 ex |
申请公布号 |
RU2330885(C2) |
申请公布日期 |
2008.08.10 |
申请号 |
RU20060110264 |
申请日期 |
2003.06.18 |
申请人 |
FEDERAL'NOE GOSUDARSTVENNOE UCHREZHDENIE NAUKI "GOSUDARSTVENNYJ NAUCHNYJ TSENTR VIRUSOLOGII I BIOTEKHNOLOGII "VEKTOR" FEDERAL'NOJ SLUZHBY PO NADZORU V SFERE ZASHCHITY PRAV POTREBITELEJ I BLAGOPOLUCHIJA CHELOVEKA (FGU GNTS VB "VEKTOR" ROSPOTREBNADZORA |
发明人 |
SANDAKHCHIEV LEV STEPANOVICH;NECHAEVA ELENA AVGUSTOVNA;VARAKSIN NIKOLAJ ANATOL'EVICH;RJABICHEVA TAT'JANA GENNAD'EVNA;MAZURKOVA NATAL'JA ALEKSEEVNA;MARKUSHIN STANISLAV GEORGIEVICH;GENDON JURIJ ZAKHAROVICH;KOPTJAEVA IRINA BORISOVNA;AKOPOVA IRINA IVANOVNA |
分类号 |
C12N7/00 |
主分类号 |
C12N7/00 |
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