METHOD FOR PREPARING FUSION PROTEIN BY TRANS-SPLICING METHOD

摘要

An object to be achieved by the present invention is to provide a method that enables convenient production of a target fusion protein within a short time without constructing any expression vector. The present invention provides a method for producing a fusion protein comprising a first protein and a second protein, which comprises the steps of: introducing a nucleic acid molecule (pre-trans-splicing molecule) or a vector capable of expressing such a nucleic acid molecule into a cell expressing the first protein, wherein the nucleic acid molecule contains a gene sequence encoding the second protein and a sequence which is capable of inducing trans-splicing through binding to pre-mRNA that is generated from a region containing a gene sequence that encodes the first protein; and recovering a fusion protein comprising the first protein and the second protein generated within the cell.