| 摘要 |
It is intended to provide a measurement method for HbA1c capable of maintaining a measurement accuracy equivalent to that immediately after blood collection even if a whole blood specimen after storage is used. The whole blood is stored in the presence of a glycolysis inhibitor, and by adding protease to the whole blood specimen after storage, hemoglobin in the whole blood specimen is cleaved. Then, fructosyl amine oxidase is allowed to act on a glycated portion of the resulting hemoglobin fragments, and redox reaction between the glycated portion and fructosyl amine oxidase is measured, whereby a glycation ratio of HbA1c is determined. Further, in place of storage of whole blood in the presence of a glycolysis inhibitor, a strong electrolyte such as KCl, K<SUB>2</SUB>SO<SUB>4</SUB>, KNO, NaCl, Na<SUB>2</SUB>SO<SUB>4</SUB>, NaNO, MgCl<SUB>2</SUB>, MgSO<SUB>4</SUB>, or Mg(NO)<SUB>2</SUB> is added to the whole blood after storage and the whole blood is subjected to a protease treatment in the presence of the strong electrolyte. According to this method, variation of HbA1c measurement values due to storage of whole blood can be avoided. |
