CHIMERIC Fc? RECEPTOR AND METHOD FOR DETERMINATION OF ADCC ACTIVITY BY USING THE RECEPTOR

摘要

Disclosed are: a chimeric receptor comprising an extracellular domain of mouse Fc? receptor and a transmembrane domain of human Fc? receptor or a chimeric receptor comprising an extracellular domain of mouse Fc? receptor and a transmembrane domain of human ?-chain; a method for the determination of the ADCC activity of a mouse antibody by using the chimeric receptor; and a method for the screening of a mouse antibody having an ADCC activity. A chimeric molecule is produced, which comprises an extracellular domain of mouse Fc? R3 or mouse Fc? R4 fused to a transmembrane domain and an intracellular domain of human ? chain or human Fc? R3. The chimeric molecule is expressed in a human NK92 cell. As a result, it is found that the chimeric receptors of both of the combinations can induce the ADCC activity. Therefore, it is found that the chimeric receptors enable to determine the ADCC activity of a mouse antibody.