Methods of using FET labeled oligonucleotides that include a 3' 5' exonuclease resistant quencher domain and compositions for practicing the same

摘要

Methods and compositions are provided for detecting a primer extension product in a reaction mixture. In the subject methods, a primer extension reaction is conducted in the presence of a polymerase having 3'†’5' exonuclease activity and at least one FET labelled oligonucleotide probe that includes a 3'†’5' exonuclease resistant quencher domain. Also provided are systems and kits for practicing the subject methods. The subject invention finds use in a variety of different applications, and are particularly suited for use in high fidelity PCR based reactions, including SNP detection applications, allelic variation detection applications, and the like.