发明名称 |
METHOD FOR MONITORING HYDROLYTIC ACTIVITY |
摘要 |
<p>The present invention relates to methods of measuring the activity of a hydrolytic agent comprising contacting a biomolecule with a hydrolytic agent in the presence of a fluorescent dye under conditions that allow digestion of the biomolecule by the hydrolytic agent. The fluorescence of the dye is monitored over time and a change in fluorescence signifies digestion of the biomolecule by the hydrolytic agent. The biomolecule is preferably a protein, peptide or proteome but can also be a carbohydrate, oligonucleotide or lipid. Further methods relate to determining an end point for digestion of a biomolecule by a hydrolytic agent, and methods of monitoring digestion of a biomolecule by a hydrolytic agent. The monitoring can be performed on the reaction mixture in real time or via sampling. The invention also relates to kits for carrying out the method.</p> |
申请公布号 |
EP1952148(A1) |
申请公布日期 |
2008.08.06 |
申请号 |
EP20060804472 |
申请日期 |
2006.11.03 |
申请人 |
FLUOROTECHNICS PTY LIMITED |
发明人 |
KARUSO, PETER, HELMUTH;CHOI, HUNG-YOON |
分类号 |
G01N33/52;C12Q1/37;G01N21/64;G01N33/58;G01N33/68 |
主分类号 |
G01N33/52 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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