| 摘要 |
In preserving biological liquids, e.g. blood, body fluids, suspensions of micro-organisms such as Azobacter, Micrococcus, Aspergillus, Escherichia, Saccharomyces, orange juice, beer, wine, droplets of the liquid are introduced into a moving stream of an inert liquid refrigerant, e.g. liquid nitrogen or other inert gas, air, nitrous oxide, halogenated hydrocarbons, having a temperature below the critical zone of the biological liquid. The frozen droplets are stored in a refrigerant, e.g. liquid nitrogen, below - 130 DEG C. before being thawed for use. The refrigerant stream is preferably below - 75 DEG C., from 0.125 to 3 inches deep and moving at 26 to 4,000 mm. per second either gravitating in a downwardly cascading or spiralling direction, or moving as a result of centrifugal impetus; suitable forms of apparatus and storage containers for the frozen droplets are described. Blood to be preserved preferably contains anticoagulant, e.g. citrate-dextrose or heparen solution, and a protective, e.g. 2.5 to 8.5% of glucose or 11% of lactose, by weight, and is stored for 1 to 48 hours at between 4 DEG to 37 DEG C. before freezing; the frozen droplets are stored below - 196 DEG C. Red blood cells (separated from plasma) are suspended for droplets freezing in polyvinyl pyrollidone solution and stored below -130 DEG C. Frozen droplets of beer, orange juice and sherry may be lyophilized after freezing. The droplets are thawed by introduction into a column of steam or an aqueous bath isotonic saline solution maintained at 37 DEG to 47 DEG C. which may contain 1 to 10% of polyvinyl pyrollidone, suitable forms of apparatus being described. |
