| 摘要 |
A method for purifying a gluconate dehydratase is provided to develop a novel gluconate dehydratase which retains activity at high temperature for a prolonged time. A method for preparing a 2-keto-3-deoxy aldonic acid is provided to produce the 2-keto-3-deoxy aldonic acid from aldonic acid efficiently by using the gluconate dehydratase. A method for purifying a gluconate dehydratase comprises a step of conducting chromatography of a culture solution or a cell from a gluconate dehydratase producing microorganism through a column packed with resin attached to at least one functional group selected from the group consisting of carboxy, carboxymethyl, sulpho, sulphomethyl, sulphoprophyl, aminoethyl, diethylaminoethyl, trimethylaminomethyl, triethylaminoethyl, dimethyl-2-hydroxyethylaminomethyl, diethyl-2-hydroxypropylaminoethyl, phospho, alkyl and hyroxylapatite, wherein the matrix of the resin is selected from the group consisting of agarose, cellulose, dextran, polyacrylate, and polystyrene and the microorganism is selected from the group consisting of Sulfolobus solfataricus, Sulfolobus acidocaldarius, Sulfolobus shibatae, Sulfolobus tokodaii, Sulfolobus metallicus, Sulfolobus hakonensis, Sulfolobus brierleyi, Sulfolobus islandicus, Sulfolobus tengchongensis, Sulfolobus thuringiensis, Sulfolobus yangmingensis, Sulfolobus sp., Thermoplasma acidophilum, Thermoplasma volcanium, and Ferroplasma acidophilum. To produce a 2-keto-3-deoxy aldonic acid from aldonic acid, the gluconate dehydratase is contacted to the to aldonic acid in water or an aqueous solvent at a temperature of 0-120 deg.C under pH of 1.5-12, wherein a mixing ratio of the gluconate dehydratase to the aldonic acid is 1mug:0.01-1 mol. |
