| 摘要 |
A method for producing butanol using bacteria capable of biosynthesizing butanol is provided to generate butyryl-CoA through various methods and obtain the butyryl-CoA using the generated butyryl-CoA as an intermediate. A method for producing butanol comprises the steps of: (a) culturing a recombinant bacterium including a gene coding an enzyme(AdhE), which converts butyryl-CoA into butanol, and into which a gene coding for CoAT (acetyl-CoA:butyryl-CoA transferase) is introduced in a butyrate or acetoacetate-containing culture medium to produce butanol; and (b) recovering the butanol from the culture solution. A recombinant bacterium having butanol producing ability is characterized in that genes coding thiolase(THL), 3-hydroxybutyryl-CoA dehydrogenase(BHBD), crotonase(CRO) and functional BCD(butyryl-CoA dehydrogenase) are introduced thereinto.
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